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image acquisition 4 5 software  (Cellular Technology Ltd)


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    Structured Review

    Cellular Technology Ltd image acquisition 4 5 software
    Image Acquisition 4 5 Software, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 99/100, based on 2108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+acquisition+4+5+software/ImmunoSpot+Software/pm41299178-502-32-36
    Average 99 stars, based on 2108 article reviews
    image acquisition 4 5 software - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Diverse priming outcomes under conditions of very rare precursor B cells.
    Article Snippet: .. The reaction was stopped by washing the plates with running tap water, and air-dried plates before imaging using the Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). .. Antibodies/ Reagents Clone Source Catalog # Dilution Fc Block - BioLegend 422302 1/20 Mouse anti-human CD40 HB14 Miltenyi 130-094-133 1:200 Mouse anti-human CXCR5 PE-Cy7 MU5UBEE Thermo Fisher Scientific 25-9185-42 1:100 Mouse anti-human CCR7 BV650 G043H7 BioLegend 353233 1:100 Mouse anti-human CD69 PE-Cy5 FN50 BioLegend 310908 1:250 Mouse anti-human CD137 (4-1BB) BV421 4B4-1 BioLegend 309819 1:250 Mouse anti-human CD25 BV605 BC96 BioLegend 302631 1:250 Mouse anti-human CD40L BB515 24–31 BD Biosciences 568170 1:250 Mouse anti-human CD134 (OX40) PE L106 BD Biosciences 340420 1:250 Mouse anti-human CD8 BUV496 RPA-T8 BD Biosciences 612943 1:100 Mouse anti-human CD14 APC-Cy7 M5E2 BioLegend 301820 1:100 Mouse anti-human CD16 APC-eFluor780 eBioCB16 Thermo Fisher Scientific 47-0168-42 1:100 Mouse anti-human CD20 APC-Cy7 2H7 BioLegend 302314 1:100 Mouse anti-human CD3 BUV395 SP34-2 BD Biosciences 564117 1:100 Mouse anti-human CD4 PerCP-Cy5.5 OKT4 BioLegend 317428 1:100 Mouse anti-human PD-1 BV785 EH12.2H7 BioLegend 329929 1:100 Mouse anti-human CD45RA PE-CF594 5H9 BD Biosciences 565419 1:100 Armenian Hamster anti-ICOS BV480 C398.4A BD Biosciences 566087 1:100 Mouse anti-human IFN-g BV750 B27 BD Biosciences 566357 1:100 Rat anti-human IL-2 BUV737 MQ1-17H12 BD Biosciences 612836 1:200 Mouse anti-human TNF-a BV711 MAb11 BioLegend 502940 1:200 Mouse anti-human Granzyme B Alexa Fluor 700 GB11 BD Biosciences 560213 1:1000 Mouse anti-human IL-21 Alexa Fluor 647 3A3-N2.1 BD Biosciences 560493 1:200 Reagent Clone Catalog # Dilution AF647 Streptavidin - BioLegend (405237) - BV421 Streptavidin - BioLegend (405225) - PE Streptavidin - BioLegend (405245) - Viability APC-eFluor506 - Thermo Fisher Scientific (65-0866-18) 1:500 CD8a APC-eFluor780 RPA-T8 BD Biosciences (563256) 1:100 (Continued on next page) e8 Immunity 58, 1–18.e1–e11, April 8, 2025

    Article Title: iHALT unlocks liver functionality as a surrogate secondary lymphoid organ
    Article Snippet: Following 16 h of incubation, plates were washed and detected for mouse with anti-Fcγ horseradish peroxidase (HRP) (Jackson) or for human with anti-IgG biotin (Mabtech) and streptavidin-HRP (Mabtech). .. Plates were developed with ELISpot TMB substrate (Mabtech) for 7 min, quenched and washed with deionized water, and left to dry in the dark overnight until imaging with Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). .. Serum IgG was determined following the coating of ELISA plates with anti-mouse H+L IgG antibody (Jackson Immuno) at 4 °C overnight.

    Article Title: Diverse priming outcomes under conditions of very rare precursor B cells
    Article Snippet: .. The reaction was stopped by washing the plates with running tap water, and air-dried plates before imaging using the Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). ..

    Article Title: iHALT unlocks liver functionality as a surrogate secondary lymphoid organ.
    Article Snippet: Following 16 h of incubation, plates were washed and detected for mouse with anti-Fcγ horseradish peroxidase (HRP) ( Jackson) or for human with anti-IgG biotin (Mabtech) and streptavidin-HRP (Mabtech). .. Plates were developed with ELISpot TMB substrate (Mabtech) for 7 min, quenched and washed with deionized water, and left to dry in the dark overnight until imaging with Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). .. Serum IgG was determined following the coating of ELISA plates with anti-mouse H+L IgG antibody ( Jackson Immuno) at 4 °C overnight.

    Software:

    Article Title: Diverse priming outcomes under conditions of very rare precursor B cells.
    Article Snippet: .. The reaction was stopped by washing the plates with running tap water, and air-dried plates before imaging using the Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). .. Antibodies/ Reagents Clone Source Catalog # Dilution Fc Block - BioLegend 422302 1/20 Mouse anti-human CD40 HB14 Miltenyi 130-094-133 1:200 Mouse anti-human CXCR5 PE-Cy7 MU5UBEE Thermo Fisher Scientific 25-9185-42 1:100 Mouse anti-human CCR7 BV650 G043H7 BioLegend 353233 1:100 Mouse anti-human CD69 PE-Cy5 FN50 BioLegend 310908 1:250 Mouse anti-human CD137 (4-1BB) BV421 4B4-1 BioLegend 309819 1:250 Mouse anti-human CD25 BV605 BC96 BioLegend 302631 1:250 Mouse anti-human CD40L BB515 24–31 BD Biosciences 568170 1:250 Mouse anti-human CD134 (OX40) PE L106 BD Biosciences 340420 1:250 Mouse anti-human CD8 BUV496 RPA-T8 BD Biosciences 612943 1:100 Mouse anti-human CD14 APC-Cy7 M5E2 BioLegend 301820 1:100 Mouse anti-human CD16 APC-eFluor780 eBioCB16 Thermo Fisher Scientific 47-0168-42 1:100 Mouse anti-human CD20 APC-Cy7 2H7 BioLegend 302314 1:100 Mouse anti-human CD3 BUV395 SP34-2 BD Biosciences 564117 1:100 Mouse anti-human CD4 PerCP-Cy5.5 OKT4 BioLegend 317428 1:100 Mouse anti-human PD-1 BV785 EH12.2H7 BioLegend 329929 1:100 Mouse anti-human CD45RA PE-CF594 5H9 BD Biosciences 565419 1:100 Armenian Hamster anti-ICOS BV480 C398.4A BD Biosciences 566087 1:100 Mouse anti-human IFN-g BV750 B27 BD Biosciences 566357 1:100 Rat anti-human IL-2 BUV737 MQ1-17H12 BD Biosciences 612836 1:200 Mouse anti-human TNF-a BV711 MAb11 BioLegend 502940 1:200 Mouse anti-human Granzyme B Alexa Fluor 700 GB11 BD Biosciences 560213 1:1000 Mouse anti-human IL-21 Alexa Fluor 647 3A3-N2.1 BD Biosciences 560493 1:200 Reagent Clone Catalog # Dilution AF647 Streptavidin - BioLegend (405237) - BV421 Streptavidin - BioLegend (405225) - PE Streptavidin - BioLegend (405245) - Viability APC-eFluor506 - Thermo Fisher Scientific (65-0866-18) 1:500 CD8a APC-eFluor780 RPA-T8 BD Biosciences (563256) 1:100 (Continued on next page) e8 Immunity 58, 1–18.e1–e11, April 8, 2025

    Article Title: Influenza vaccine based on AS03-adjuvanted chimeric HA induces long-lived stalk-specific plasma cells in bone marrow and lymph nodes of nonhuman primates
    Article Snippet: .. Once dried, the plates were analyzed with the Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology) until week 94. .. Plates after week 94 were read by a Mabtech IRIS fluorospot/ELISpot reader.

    Article Title: iHALT unlocks liver functionality as a surrogate secondary lymphoid organ
    Article Snippet: Following 16 h of incubation, plates were washed and detected for mouse with anti-Fcγ horseradish peroxidase (HRP) (Jackson) or for human with anti-IgG biotin (Mabtech) and streptavidin-HRP (Mabtech). .. Plates were developed with ELISpot TMB substrate (Mabtech) for 7 min, quenched and washed with deionized water, and left to dry in the dark overnight until imaging with Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). .. Serum IgG was determined following the coating of ELISA plates with anti-mouse H+L IgG antibody (Jackson Immuno) at 4 °C overnight.

    Article Title: Chimeric hemagglutinin-based universal influenza mRNA vaccine induces protective immunity and bone marrow plasma cells in rhesus macaques
    Article Snippet: .. The plates were dried and detected using the Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). ..

    Article Title: Diverse priming outcomes under conditions of very rare precursor B cells
    Article Snippet: .. The reaction was stopped by washing the plates with running tap water, and air-dried plates before imaging using the Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). ..

    Article Title: iHALT unlocks liver functionality as a surrogate secondary lymphoid organ.
    Article Snippet: Following 16 h of incubation, plates were washed and detected for mouse with anti-Fcγ horseradish peroxidase (HRP) ( Jackson) or for human with anti-IgG biotin (Mabtech) and streptavidin-HRP (Mabtech). .. Plates were developed with ELISpot TMB substrate (Mabtech) for 7 min, quenched and washed with deionized water, and left to dry in the dark overnight until imaging with Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). .. Serum IgG was determined following the coating of ELISA plates with anti-mouse H+L IgG antibody ( Jackson Immuno) at 4 °C overnight.

    Article Title: Influenza vaccine based on AS03-adjuvanted chimeric HA induces long-lived stalk-specific plasma cells in bone marrow and lymph nodes of nonhuman primates.
    Article Snippet: .. Once dried, the plates were analyzed with the Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology) until week 94. .. Plates after week 94 were read by a Mabtech IRIS fluorospot/ELISpot reader.

    Article Title: Chimeric hemagglutinin-based universal influenza mRNA vaccine induces protective immunity and bone marrow plasma cells in rhesus macaques.
    Article Snippet: .. The plates were dried and detected using the Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). ..

    Enzyme-linked Immunospot:

    Article Title: iHALT unlocks liver functionality as a surrogate secondary lymphoid organ
    Article Snippet: Following 16 h of incubation, plates were washed and detected for mouse with anti-Fcγ horseradish peroxidase (HRP) (Jackson) or for human with anti-IgG biotin (Mabtech) and streptavidin-HRP (Mabtech). .. Plates were developed with ELISpot TMB substrate (Mabtech) for 7 min, quenched and washed with deionized water, and left to dry in the dark overnight until imaging with Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). .. Serum IgG was determined following the coating of ELISA plates with anti-mouse H+L IgG antibody (Jackson Immuno) at 4 °C overnight.

    Article Title: iHALT unlocks liver functionality as a surrogate secondary lymphoid organ.
    Article Snippet: Following 16 h of incubation, plates were washed and detected for mouse with anti-Fcγ horseradish peroxidase (HRP) ( Jackson) or for human with anti-IgG biotin (Mabtech) and streptavidin-HRP (Mabtech). .. Plates were developed with ELISpot TMB substrate (Mabtech) for 7 min, quenched and washed with deionized water, and left to dry in the dark overnight until imaging with Immunospot CTL counter and Image Acquisition 4.5 software (Cellular Technology). .. Serum IgG was determined following the coating of ELISA plates with anti-mouse H+L IgG antibody ( Jackson Immuno) at 4 °C overnight.



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